Flow Chart for rDNA Hepatitis B Vaccine Preparation

 Creating a flow chart for the preparation of a recombinant DNA (rDNA) hepatitis B vaccine involves several key steps. Below is a simplified overview of the process:

Flow Chart for rDNA Hepatitis B Vaccine Preparation

1. Gene Cloning

   • Isolate the gene encoding hepatitis B surface antigen (HBsAg) from the hepatitis B virus.
   • Insert the HBsAg gene into a plasmid vector.

2. Transformation

   • Introduce the recombinant plasmid into a host organism (commonly E. coli or yeast).
   • Select transformed cells using antibiotic resistance markers.

3. Expression

   • Grow the transformed host cells in a suitable culture medium.
   • Induce the expression of the HBsAg protein.

4. Harvesting

   • Collect the cultured cells and lyse them to release the HBsAg protein.
   • Centrifuge to separate cellular debris from the supernatant.

5. Purification

   • Purify the HBsAg protein using techniques such as affinity chromatography.
   • Validate the purity and activity of the protein.

6. Formulation

   • Formulate the purified HBsAg into a vaccine by adding adjuvants and stabilizers.
   • Prepare the vaccine for storage and distribution.

7. Quality Control

   • Conduct tests for potency, safety, and sterility.
   • Ensure compliance with regulatory standards.

8. Packaging and Distribution

   • Package the vaccine in suitable vials or syringes.
   • Distribute to healthcare facilities.